Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/11095
Title: Expression and function of ATIP/MTUS1 in human prostate cancer cell lines.
Authors: Louis, Simon N S;Chow, Laurie T C;Rezmann, Linda Adriana;Krezel, Michael A;Catt, Kevin J;Tikellis, Christos;Frauman, Albert G;Louis, William J
Affiliation: Clinical Pharmacology and Therapeutics Unit, University of Melbourne, Department of Medicine, Austin Health, Heidelberg, Victoria, Australia.
Issue Date: 1-Oct-2010
Citation: The Prostate; 70(14): 1563-74
Abstract: We have previously demonstrated Ang II type 2 (AT(2)-) receptor-mediated inhibition of EGF-induced prostate cancer cell growth in androgen-dependent (LNCaP) and independent (PC3) prostate cancer cell lines.To explore the signaling pathways involved in this inhibitory effect, we examined the interaction of the AT(2)-receptor with its novel regulatory partner ATIP using real time PCR, over-expression, siRNA and [(3)H]thymidine incorporation assays.The results in human prostate cancer cell lines demonstrate the presence of ATIP in both cell lines examined, and suggest that (i) the AT(2)-receptor through an interaction with ATIP mediates an anti-growth factor effect in both androgen-dependent and androgen-independent cell lines; (ii) ATIP expression decreases as the rate of cell growth and androgen-independence increase; and (iii) EGF may act on cell growth in part by reducing the content of ATIP present in the cells.The results support our earlier proposal in normal cell lines that ATIP is an important component of the cellular response to AT(2)-receptor activation. The results further suggest that a critical level of ATIP is required to mediate the effect of AT(2)-receptor activation to inhibit EGF mediated increases in cell growth. They also suggest that EGF may in part induce cell growth by suppressing the level of ATIP expression.
Internal ID Number: 20687230
URI: http://ahro.austin.org.au/austinjspui/handle/1/11095
DOI: 10.1002/pros.21192
URL: http://www.ncbi.nlm.nih.gov/pubmed/20687230
Type: Journal Article
Subjects: Cell Line, Tumor
DNA Primers
Epidermal Growth Factor.pharmacology
Gene Expression Regulation, Neoplastic
Humans
Male
Polymerase Chain Reaction
Prostatic Neoplasms.chemically induced.genetics.metabolism
RNA, Messenger.genetics
RNA, Small Interfering.genetics
Thymidine.metabolism
Tumor Suppressor Proteins.genetics.metabolism
Appears in Collections:Journal articles

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