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Title: | Specific binding of D-Tyr25 (Nle28,31)-CCK(25-33) to cortical membranes from rat brain. |
Authors: | Carlberg, M;Jarrott, B;Beart, P M |
Affiliation: | University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Austin Hospital, Heidelberg, Vic. |
Issue Date: | 14-Jan-1991 |
Citation: | Neuroscience Letters; 122(1): 29-32 |
Abstract: | The 125I-labeled sulfonated cholecystokinin (CCK) analogue, D-Tyr25(Nle28,31)-CCK(25-33), bound saturably and reversibly to membranes of rat cerebral cortex. The dissociation constant Kd was estimated to be 0.14 nM by Scatchard analysis and 0.40 nM from analyses of dissociation/association rates. Hill coefficients of 0.99, Scatchard plots and drug competition studies were consistent with a single population of binding sites. Dependence on divalent cations and inhibition by guanylyl-imidodiphosphate suggested binding to a receptor coupled to a G protein. Competition studies with CCK-analogues and non-peptide specific antagonists indicated binding to receptor sites of the B-subtype (CCKB). |
Internal ID Number: | 2057133 |
URI: | http://ahro.austin.org.au/austinjspui/handle/1/11061 |
URL: | http://www.ncbi.nlm.nih.gov/pubmed/2057133 |
Type: | Journal Article |
Subjects: | Animals Benzodiazepinones.metabolism Binding, Competitive Cerebral Cortex.metabolism Cholecystokinin.analogs & derivatives.metabolism Half-Life In Vitro Techniques Iodine Radioisotopes.diagnostic use Kinetics Male Membranes.metabolism Peptide Fragments.metabolism Phenylurea Compounds Rats Rats, Inbred Strains Receptors, Cholecystokinin.antagonists & inhibitors.metabolism |
Appears in Collections: | Journal articles |
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