Please use this identifier to cite or link to this item:
|Title:||Specific binding of D-Tyr25 (Nle28,31)-CCK(25-33) to cortical membranes from rat brain.|
|Authors:||Carlberg, M;Jarrott, B;Beart, P M|
|Affiliation:||University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Austin Hospital, Heidelberg, Vic.|
|Citation:||Neuroscience Letters; 122(1): 29-32|
|Abstract:||The 125I-labeled sulfonated cholecystokinin (CCK) analogue, D-Tyr25(Nle28,31)-CCK(25-33), bound saturably and reversibly to membranes of rat cerebral cortex. The dissociation constant Kd was estimated to be 0.14 nM by Scatchard analysis and 0.40 nM from analyses of dissociation/association rates. Hill coefficients of 0.99, Scatchard plots and drug competition studies were consistent with a single population of binding sites. Dependence on divalent cations and inhibition by guanylyl-imidodiphosphate suggested binding to a receptor coupled to a G protein. Competition studies with CCK-analogues and non-peptide specific antagonists indicated binding to receptor sites of the B-subtype (CCKB).|
|Internal ID Number:||2057133|
Cholecystokinin.analogs & derivatives.metabolism
In Vitro Techniques
Iodine Radioisotopes.diagnostic use
Rats, Inbred Strains
Receptors, Cholecystokinin.antagonists & inhibitors.metabolism
|Appears in Collections:||Journal articles|
Files in This Item:
There are no files associated with this item.
Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.