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dc.contributor.authorIerino, Francesco Len
dc.contributor.authorMulley, Williamen
dc.contributor.authorDodge, Natalieen
dc.contributor.authorLi, Yu Qinen
dc.contributor.authorMouhtouris, Effieen
dc.contributor.authorChristiansen, Daleen
dc.contributor.authorSandrin, Mauro Sen
dc.date.accessioned2015-05-16T00:35:13Z
dc.date.available2015-05-16T00:35:13Z
dc.date.issued2010-04-20en
dc.identifier.citationImmunology and Cell Biology 2010; 88(8): 846-50en
dc.identifier.govdoc20404834en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/11008en
dc.description.abstractDendritic cells (DCs) and CTLA4Ig are important in regulating T-cell responses and therefore represent potential therapeutic tools in transplantation. In this study, CTLA4Ig was expressed in a C57BL/6 murine DC line (JAWS II) by lentiviral transduction and these cells were used to examine T-cell immunomodulatory effects in vitro and in vivo. A lower stimulation index to C57BL/6 was observed with splenocytes from BALB/c mice primed with JAWS II-CTLA4Ig compared with control JAWS II-green fluorescent protein (JAWS II-GFP). Mice primed with JAWS II-CTLA4Ig cells had significantly prolonged antigen-specific C57BL/6 skin graft survival compared with either JAWS II-GFP-primed or naïve mice (median 13, 11 and 11 days, respectively, P=0.0001). Furthermore, JAWS II-CTLA4Ig-primed mice that had been previously transplanted with skin grafts were re-transplanted with skin grafts 6 months later without immune manipulation. These mice demonstrated specific prolongation of second-set rejection responses, indicating systemic immune modulation induced by genetically modified DC. The mechanism was not due to expression of indoleamine 2,3-dioxygenase or induction of circulating regulatory T cells as assessed by flow cytometry of the peripheral blood lymphocytes. This potent effect demonstrated with skin grafts and second-set responses highlights the potential use of this strategy for transplantation more generally.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherCell Growth Processes.genetics.immunologyen
dc.subject.otherCell Lineen
dc.subject.otherDendritic Cells.immunology.metabolism.pathologyen
dc.subject.otherGraft Survival.geneticsen
dc.subject.otherImmunoconjugates.genetics.immunology.metabolismen
dc.subject.otherImmunologic Memory.geneticsen
dc.subject.otherLymphocyte Activation.geneticsen
dc.subject.otherLymphocyte Culture Test, Mixeden
dc.subject.otherMiceen
dc.subject.otherMice, Inbred BALB Cen
dc.subject.otherMice, Inbred C57BLen
dc.subject.otherSkin Transplantationen
dc.subject.otherT-Lymphocytes.immunology.metabolism.pathologyen
dc.subject.otherTransgenes.geneticsen
dc.titleDendritic cells expressing soluble CTLA4Ig prolong antigen-specific skin graft survival.en
dc.typeJournal Articleen
dc.identifier.journaltitleImmunology and cell biologyen
dc.identifier.affiliationDepartment of Nephrology, Austin Health, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1038/icb.2010.58en
dc.description.pages846-50en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/20404834en
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