Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10839
Title: Regulation of the 5'-flanking region of the human CYP27B1 gene in osteoblast cells.
Authors: Turner, A G;Dwivedi, P P;Anderson, Paul H;May, B K;Morris, Howard A
Affiliation: Department of Medicine, Austin Health, University of Melbourne, Australia. atur@unimelb.edu.au
Issue Date: 11-Jun-2009
Citation: Molecular and Cellular Endocrinology 2009; 311(1-2): 55-61
Abstract: Synthesis of 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) is catalysed by the enzyme 25-hydroxyvitamin D(3)-1alpha-hydroxylase (CYP27B1). Regulation of CYP27B1 gene expression is poorly understood, particularly in non-renal tissues including bone where 1,25(OH)(2)D(3) is hypothesised to serve autocrine/paracrine roles. Transient transfection of ROS 17/2.8 osteoblast-like cells with reporter gene constructs containing deletions of the 5'-flanking region of the human CYP27B1 gene revealed a proximal promoter, enhancer region and strong upstream repressive region. Putative CCAAT and GC boxes, as well as Ets protein binding sites were shown to contribute to promoter and enhancer activities respectively in common with kidney and prostate cells. Inhibition of basal expression was largely attributed to a palindrome 5'-GTCTCAGAC-3' (-1015/-1007bp) that contains two putative canonical Smad binding elements. We conclude that repression of CYP27B1 gene expression may be a common event but the novel inhibitory elements we have identified may be unique to osteoblasts.
Internal ID Number: 19524013
URI: http://ahro.austin.org.au/austinjspui/handle/1/10839
DOI: 10.1016/j.mce.2009.06.001
URL: http://www.ncbi.nlm.nih.gov/pubmed/19524013
Type: Journal Article
Subjects: 25-Hydroxyvitamin D3 1-alpha-Hydroxylase.genetics
5' Flanking Region.genetics
Animals
Base Sequence
Cell Line, Tumor
DNA Mutational Analysis
Enhancer Elements, Genetic.genetics
Gene Expression Regulation.drug effects
Humans
Molecular Sequence Data
Osteoblasts.drug effects.enzymology
Promoter Regions, Genetic.genetics
Rats
Sequence Deletion
Transforming Growth Factor beta.pharmacology
Appears in Collections:Journal articles

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