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Title: Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research.
Authors: Lubel, J S;Herath, C B;Velkoska, Elena;Casley, David J;Burrell, Louise M;Angus, Peter W
Affiliation: Department of Medicine, Austin and Northern Health, Repatriation Hospital, University of Melbourne, Waterdale Road, Heidelberg Heights, Melbourne, VIC, Australia,
Issue Date: 18-Feb-2009
Citation: Cytotechnology 2009; 58(3): 119-26
Abstract: Cell culture experiments often employ the use of culture media that contain fetal calf serum (FCS). The angiotensin peptides angiotensin II and angiotensin 1-7 have opposing effects with angiotensin converting enzyme 2 (ACE2) being the enzyme predominantly responsible for generating angiotensin 1-7 from angiotensin II. The effect of FCS on angiotensin peptides has not previously been described. We have shown that FCS has ACE2 enzyme activity capable of degrading angiotensin II and generating angiotensin 1-7. Researchers should be aware that FCS possesses ACE2 activity and that heat-treating FCS to 56 degrees C only partially inhibits this enzyme activity, whereas heat-treating to 70 degrees C completely abolishes ACE2 activity.
Internal ID Number: 19224387
DOI: 10.1007/s10616-009-9185-0
Type: Journal Article
Appears in Collections:Journal articles

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