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Title: Cancer/testis antigens can be immunological targets in clonogenic CD133+ melanoma cells.
Authors: Gedye, Craig;Quirk, Juliet;Browning, Judy;Svobodov√°, Suzanne;John, Thomas;Sluka, Pavel;Dunbar, P Rod;Corbeil, Denis;Cebon, Jonathan S;Davis, Ian D
Ludwig Institute for Cancer Research, Austin Hospital, Studley Road, Heidelberg, VIC, 3084, Australia
Issue Date: 17-Feb-2009
Citation: Cancer Immunology, Immunotherapy : Cii 2009; 58(10): 1635-46
Abstract: "Cancer stem cells" that resist conventional treatments may be a cause of therapeutic failure in melanoma. We report a subpopulation of clonogenic melanoma cells that are characterized by high prominin-1/CD133 expression in melanoma and melanoma cell lines. These cells have enhanced clonogenicity and self-renewal in vitro, and serve as a limited in vitro model for melanoma stem cells. In some cases clonogenic CD133(+) melanoma cells show increased expression of some cancer/testis (CT) antigens. The expression of NY-ESO-1 in an HLA-A2 expressing cell line allowed CD133(+) clonogenic melanoma cells to be targeted for killing in vitro by NY-ESO-1-specific CD8(+) T-lymphocytes. Our in vitro findings raise the hypothesis that if melanoma stem cells express CT antigens in vivo that immune targeting of these antigens may be a viable clinical strategy for the adjuvant treatment of melanoma.
Internal ID Number: 19221743
DOI: 10.1007/s00262-009-0672-0
Type: Journal Article
Subjects: Antigens, CD.metabolism
Antigens, Neoplasm.immunology
CD8-Positive T-Lymphocytes.immunology
Cancer Vaccines.therapeutic use
Colony-Forming Units Assay
Cytotoxicity, Immunologic
Fluorescent Antibody Technique
HLA-A2 Antigen.genetics.immunology
Immunoenzyme Techniques
Lymphatic Metastasis
Membrane Proteins.immunology
Peptide Fragments.immunology
RNA, Messenger.genetics.metabolism
Reverse Transcriptase Polymerase Chain Reaction
Skin Neoplasms.immunology.pathology.therapy
T-Lymphocytes, Cytotoxic.immunology
Appears in Collections:Journal articles

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