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dc.contributor.authorHaase, Men
dc.contributor.authorBellomo, Rinaldoen
dc.contributor.authorBaldwin, Ianen
dc.contributor.authorHaase-Fielitz, Anjaen
dc.contributor.authorStorr, Men
dc.contributor.authorKrause, Ben
dc.contributor.authorBoyce, Neilen
dc.contributor.authorSvobodova, Sen
dc.contributor.authorLi, Wen
dc.contributor.authorBagshaw, Sean Men
dc.contributor.authorWarrillow, Stephen Jen
dc.contributor.authorLangenberg, Christophen
dc.contributor.authorMorgera, Stanislaoen
dc.identifier.citationThe International Journal of Artificial Organs; 31(8): 722-9en
dc.description.abstractA novel type of adsorptive plasma filtering device (ETX-A) capable of removing endotoxin from blood in a single step has recently been developed using nanotechnology.In a miniaturized, ex vivo model of extracorporeal circuits, we tested the capacity to reduce plasma cytokine concentration of ETX-A filters in comparison to standard high-flux (HF) filters, high cut-off (HCO) filters and a control. Blood from six healthy volunteers was spiked with endotoxin and then circulated through closed (ETX-A, control) or open (HF, HCO) circuits. Blood flow was set at 16 ml/min and filtration flow at 1 ml/min. Samples for measurement of IL-1ra and IL-6 were taken at baseline and at 4 hours.Compared to control (703.3 [850.6] pg/mL), in HCO (383.5 [1144.1] pg/mL) and ETX-A (490.1 [683.2] pg/mL) filters, plasma IL-1ra pooled pre- and postfilter concentrations were lower at the end of the experiment (P=0.002; P=0.050, respectively) whereas, in standard HF filters, IL-1ra concentration was higher than control. HCO showed a trend toward a reduced relative increase in IL-6 concentration from commencement to end of experiment compared to control (P=0.07). After pooling end-of-experiment plasma cytokine values of novel blood purification devices, we found HCO + ETX-A superior to H with regard to reduction of IL-1ra (-27.0 [-20.5]% vs. 8.1 [18.9]%; p<0.001) and IL-6 (-18.0 [38.3]% vs. -1.1 [24.3]%; P=0.050) compared to control.HCO and ETX-A appeared to significantly reduce plasma IL-1ra and, when combined, plasma IL-6 concentration as well. It appears desirable to manufacture full-size blood purification devices using this technology and to explore their effect on cytokine removal.en
dc.subject.otherEquipment Designen
dc.subject.otherInterleukin 1 Receptor Antagonist Protein.blooden
dc.subject.otherMiddle Ageden
dc.subject.otherTime Factorsen
dc.titleThe effect of three different miniaturized blood purification devices on plasma cytokine concentration in an ex vivo model of endotoxinemia.en
dc.typeJournal Articleen
dc.identifier.journaltitleInternational Journal of Artificial Organsen
dc.identifier.affiliationIntensive Care Research, Austin Health, University of Melbourne, Australiaen
Appears in Collections:Journal articles

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