Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10613
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dc.contributor.authorTrevisin, Michelleen
dc.contributor.authorPollock, Wendyen
dc.contributor.authorDimech, Wayneen
dc.contributor.authorSavige, Judy Aen
dc.date.accessioned2015-05-16T00:07:36Z
dc.date.available2015-05-16T00:07:36Z
dc.date.issued2008-04-25en
dc.identifier.citationJournal of Immunological Methods 2008; 336(2): 104-12en
dc.identifier.govdoc18499121en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/10613en
dc.description.abstractThis study compared the performance of a flow cytometric immunoassay for antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) and myeloperoxidase (MPO), with indirect immunofluorescence (IIF) and ELISAs from 12 different manufacturers. Sera were from patients with active (n=55) or treated (n=68) small vessel vasculitis, or inflammatory bowel disease (IBD, n=22). The immunoassay specificity was 88% compared with 96% for IIF and 94% (median, range 91-96%) for both ELISAs. Its sensitivity in treated disease was 82% compared with 84% for IIF and 69% (median, range 57-82%) for the ELISAs. The immunoassay's specificity was 88% which was the same as the median for both ELISAs (range 84-95%). The PR3- and MPO-ANCA immunoassay was almost as sensitive as IIF, and more sensitive than, but just as specific as, most ELISAs, in detecting ANCA in active and treated vasculitis. A major advantage of this assay is its ability to be further modified to simultaneously screen for a panel of autoantibodies relevant to vasculitis.en
dc.language.isoenen
dc.subject.otherAntibodies, Antineutrophil Cytoplasmic.blooden
dc.subject.otherEnzyme-Linked Immunosorbent Assay.methodsen
dc.subject.otherFlow Cytometry.methodsen
dc.subject.otherFluorescent Antibody Technique, Indirect.methodsen
dc.subject.otherHumansen
dc.subject.otherImmunoassay.methodsen
dc.subject.otherInflammatory Bowel Diseases.blood.diagnosisen
dc.subject.otherMyeloblastin.blooden
dc.subject.otherPeroxidase.blooden
dc.subject.otherReproducibility of Resultsen
dc.subject.otherSensitivity and Specificityen
dc.subject.otherVasculitis.blood.diagnosisen
dc.titleEvaluation of a multiplex flow cytometric immunoassay to detect PR3- and MPO-ANCA in active and treated vasculitis, and in inflammatory bowel disease (IBD).en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of immunological methodsen
dc.identifier.affiliationPathology Department, Austin Health, Heidelberg, VIC 3084, Australiaen
dc.identifier.doi10.1016/j.jim.2008.03.012en
dc.description.pages104-12en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/18499121en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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