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https://ahro.austin.org.au/austinjspui/handle/1/10613
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Trevisin, Michelle | en |
dc.contributor.author | Pollock, Wendy | en |
dc.contributor.author | Dimech, Wayne | en |
dc.contributor.author | Savige, Judy A | en |
dc.date.accessioned | 2015-05-16T00:07:36Z | |
dc.date.available | 2015-05-16T00:07:36Z | |
dc.date.issued | 2008-04-25 | en |
dc.identifier.citation | Journal of Immunological Methods 2008; 336(2): 104-12 | en |
dc.identifier.govdoc | 18499121 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/10613 | en |
dc.description.abstract | This study compared the performance of a flow cytometric immunoassay for antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) and myeloperoxidase (MPO), with indirect immunofluorescence (IIF) and ELISAs from 12 different manufacturers. Sera were from patients with active (n=55) or treated (n=68) small vessel vasculitis, or inflammatory bowel disease (IBD, n=22). The immunoassay specificity was 88% compared with 96% for IIF and 94% (median, range 91-96%) for both ELISAs. Its sensitivity in treated disease was 82% compared with 84% for IIF and 69% (median, range 57-82%) for the ELISAs. The immunoassay's specificity was 88% which was the same as the median for both ELISAs (range 84-95%). The PR3- and MPO-ANCA immunoassay was almost as sensitive as IIF, and more sensitive than, but just as specific as, most ELISAs, in detecting ANCA in active and treated vasculitis. A major advantage of this assay is its ability to be further modified to simultaneously screen for a panel of autoantibodies relevant to vasculitis. | en |
dc.language.iso | en | en |
dc.subject.other | Antibodies, Antineutrophil Cytoplasmic.blood | en |
dc.subject.other | Enzyme-Linked Immunosorbent Assay.methods | en |
dc.subject.other | Flow Cytometry.methods | en |
dc.subject.other | Fluorescent Antibody Technique, Indirect.methods | en |
dc.subject.other | Humans | en |
dc.subject.other | Immunoassay.methods | en |
dc.subject.other | Inflammatory Bowel Diseases.blood.diagnosis | en |
dc.subject.other | Myeloblastin.blood | en |
dc.subject.other | Peroxidase.blood | en |
dc.subject.other | Reproducibility of Results | en |
dc.subject.other | Sensitivity and Specificity | en |
dc.subject.other | Vasculitis.blood.diagnosis | en |
dc.title | Evaluation of a multiplex flow cytometric immunoassay to detect PR3- and MPO-ANCA in active and treated vasculitis, and in inflammatory bowel disease (IBD). | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Journal of immunological methods | en |
dc.identifier.affiliation | Pathology Department, Austin Health, Heidelberg, VIC 3084, Australia | en |
dc.identifier.doi | 10.1016/j.jim.2008.03.012 | en |
dc.description.pages | 104-12 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/18499121 | en |
dc.type.austin | Journal Article | en |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
Appears in Collections: | Journal articles |
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