Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10591
Title: The regulatory T cell-associated transcription factor FoxP3 is expressed by tumor cells.
Authors: Ebert, Lisa M;Tan, Bee Shin;Browning, Judy;Svobodova, Suzanne;Russell, Sarah E;Kirkpatrick, Naomi;Gedye, Craig;Moss, Denis;Ng, Sweet Ping;MacGregor, Duncan;Davis, Ian D;Cebon, Jonathan S;Chen, Weisan
Affiliation: Ludwig Institute for Cancer Research (Melbourne Center for Clinical Sciences), Austin Health, Heidelberg, Victoria, Australia. lisa.ebert@ludwig.edu.au
Issue Date: 15-Apr-2008
Citation: Cancer Research; 68(8): 3001-9
Abstract: FoxP3 is a member of the forkhead family of transcription factors critically involved in the development and function of CD25(+) regulatory T cells (Treg). Until recently, FoxP3 expression was thought to be restricted to the T-cell lineage. However, using immunohistochemistry and flow cytometric analysis of human melanoma tissue, we detected FoxP3 expression not only in the tumor infiltrating Treg but also in the melanoma cells themselves. FoxP3 is also widely expressed by established human melanoma cell lines (as determined by flow cytometry, PCR, and Western blot), as well as cell lines derived from other solid tumors. Normal B cells do not express FoxP3; however, expression could be induced after transformation with EBV in vitro and in vivo, suggesting that malignant transformation of healthy cells can induce FoxP3. In addition, a FOXP3 mRNA variant lacking exons 3 and 4 was identified in tumor cell lines but was absent from Treg. Interestingly, this alternative splicing event introduces a translation frame-shift that is predicted to encode a novel protein. Together, our results show that FoxP3, a key regulator of immune suppression, is not only expressed by Treg but also by melanoma cells, EBV-transformed B cells, and a wide variety of tumor cell lines.
Internal ID Number: 18413770
URI: http://ahro.austin.org.au/austinjspui/handle/1/10591
DOI: 10.1158/0008-5472.CAN-07-5664
URL: http://www.ncbi.nlm.nih.gov/pubmed/18413770
Type: Journal Article
Subjects: B-Lymphocytes.immunology.virology
Cell Line, Tumor
Flow Cytometry
Forkhead Transcription Factors.genetics.immunology
Glioma.genetics.immunology
Herpesvirus 4, Human.immunology
Humans
Lymphocyte Activation.immunology
Male
Melanoma.genetics.immunology
Prostatic Neoplasms.genetics.immunology
T-Lymphocytes, Regulatory.immunology
Appears in Collections:Journal articles

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