Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10573
Title: Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries.
Authors: Wookey, Peter J;Zulli, Anthony;Buxton, Brian F;Hare, David L
Affiliation: Departments of Cardiology, Medicine (University of Melbourne), and Cardiac Surgery, Austin Health, Heidelberg, Victoria, Australia. pwookey@unimelb.edu.au
Issue Date: 1-Apr-2008
Citation: Histopathology; 52(5): 605-12
Abstract: To determine and quantify calcitonin receptor (CTR) immunoreactivity associated with specific cell types within, and associated with, the endothelial layers, neo-intima, media and vasa vasorum of diseased radial and internal mammary arteries.Immunohistochemistry and anti-CTR antibodies were used to identify positive cells within remnants of diseased human radial (n = 3) and internal mammary arteries (n = 4) that remained after bypass surgery. Three cell types expressed CTR, including endothelial cells, fibroblast-like cells within the neo-intima, and cellular structures aligned with the smooth muscle cells of the media. Other smaller cells within the surrounding parenchyma of the vasa vasorum of diseased vessels and blood-borne cells were also immunoreactive. Immunoquantification of CTR expression (Intensity x Proportional Area) in the endothelium (P < 0.05), neo-intima (P < 0.02) and media (P < 0.03) established a significant statistical correlation (Students' two-tailed t-test) with the ratio of intimal/media thickness.Increased immunoreactivity developed using anti-CTR antibodies was associated with specific cell types in the endothelial layers, neo-intima, media and vasa vasorum of diseased regions of radial and internal mammary arteries, in which there was an increased intimal/media ratio. Furthermore, CTR+, blood-borne cells present in the vessels of diseased regions suggest recruitment into these surrounding tissues.
Internal ID Number: 18370957
URI: http://ahro.austin.org.au/austinjspui/handle/1/10573
DOI: 10.1111/j.1365-2559.2008.02979.x
URL: http://www.ncbi.nlm.nih.gov/pubmed/18370957
Type: Journal Article
Subjects: Aged
Biological Markers.metabolism
Calcinosis.metabolism.pathology
Endothelium, Vascular.metabolism.pathology
Fluorescent Antibody Technique, Indirect
Humans
Male
Mammary Arteries.metabolism.pathology
Middle Aged
Radial Artery.metabolism.pathology
Receptors, Calcitonin.metabolism
Tunica Media.metabolism.pathology
Appears in Collections:Journal articles

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