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|Title:||Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries.|
|Authors:||Wookey, Peter J;Zulli, Anthony;Buxton, Brian F;Hare, David L|
|Affiliation:||Departments of Cardiology, Medicine (University of Melbourne), and Cardiac Surgery, Austin Health, Heidelberg, Victoria, Australia|
|Citation:||Histopathology; 52(5): 605-12|
|Abstract:||To determine and quantify calcitonin receptor (CTR) immunoreactivity associated with specific cell types within, and associated with, the endothelial layers, neo-intima, media and vasa vasorum of diseased radial and internal mammary arteries.Immunohistochemistry and anti-CTR antibodies were used to identify positive cells within remnants of diseased human radial (n = 3) and internal mammary arteries (n = 4) that remained after bypass surgery. Three cell types expressed CTR, including endothelial cells, fibroblast-like cells within the neo-intima, and cellular structures aligned with the smooth muscle cells of the media. Other smaller cells within the surrounding parenchyma of the vasa vasorum of diseased vessels and blood-borne cells were also immunoreactive. Immunoquantification of CTR expression (Intensity x Proportional Area) in the endothelium (P < 0.05), neo-intima (P < 0.02) and media (P < 0.03) established a significant statistical correlation (Students' two-tailed t-test) with the ratio of intimal/media thickness.Increased immunoreactivity developed using anti-CTR antibodies was associated with specific cell types in the endothelial layers, neo-intima, media and vasa vasorum of diseased regions of radial and internal mammary arteries, in which there was an increased intimal/media ratio. Furthermore, CTR+, blood-borne cells present in the vessels of diseased regions suggest recruitment into these surrounding tissues.|
|Internal ID Number:||18370957|
Fluorescent Antibody Technique, Indirect
|Appears in Collections:||Journal articles|
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