Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10488
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dc.contributor.authorTrinder, Den
dc.contributor.authorStephenson, J Men
dc.contributor.authorGao, Xen
dc.contributor.authorPhillips, P Aen
dc.contributor.authorRisvanis, Johnen
dc.contributor.authorJohnston, Colin Ien
dc.date.accessioned2015-05-15T23:56:59Z
dc.date.available2015-05-15T23:56:59Z
dc.date.issued1991-11-12en
dc.identifier.citationPeptides; 12(6): 1195-200en
dc.identifier.govdoc1815207en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/10488en
dc.description.abstractBinding characteristics of the selective V2 antagonist radioligand [3H]desGly-NH2(9)-d(CH2)5[D-Ileu2,Ileu4]AVP to rat kidney were determined. Binding was specific, saturable and reversible. The peptide bound to a single class of high-affinity binding sites with Bmax 69.4 +/- 6.8 fmol/mg protein and KD 2.8 +/- 0.3 nM. AVP and other related peptides displaced [3H]desGly-NH2(9)-d(CH2)5[D-Ileu2,Ileu4]AVP binding. The order of potency of inhibition was desamino-D-AVP greater than AVP greater than d(CH2)5[D-Ileu2,Ileu4]AVP greater than oxytocin greater than d(CH2)5[Tyr(Me)2]AVP greater than d(CH2)5[sarcosine7]AVP, which is typical of a selective V2 radioligand. Autoradiographic localization of [3H]desGly-NH2(9)-d(CH2)5[D-Ileu2,Ileu4]AVP binding sites in kidney showed dense binding in the inner and outer medulla with less binding in the cortex, which is consistent with known renal V2 receptor distribution.en
dc.language.isoenen
dc.subject.otherAngiotensin Receptor Antagonistsen
dc.subject.otherAnimalsen
dc.subject.otherArginine Vasopressin.analogs & derivatives.metabolismen
dc.subject.otherAutoradiographyen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherKidney Medulla.metabolismen
dc.subject.otherKineticsen
dc.subject.otherMembranes.metabolismen
dc.subject.otherRadioligand Assayen
dc.subject.otherRatsen
dc.subject.otherReceptors, Angiotensin.classification.metabolismen
dc.subject.otherReceptors, Vasopressinen
dc.title[3H]desGly-NH2(9)-d(CH2)5[D-Ileu2,Ileu4]AVP: an AVP V2 receptor antagonist radioligand.en
dc.typeJournal Articleen
dc.identifier.journaltitlePeptidesen
dc.identifier.affiliationUniversity of Melbourne, Department of Medicine, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages1195-200en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/1815207en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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