Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10465
Title: Antibiotic-induced release of endotoxin in chronically bacteriuric patients.
Authors: Hurley, J C;Louis, William J;Tosolini, F A;Carlin, J B
Affiliation: Department of Clinical Pharmacology and Therapeutics, Austin Hospital, Heidelberg.
Issue Date: 1-Nov-1991
Citation: Antimicrobial Agents and Chemotherapy; 35(11): 2388-94
Abstract: A novel in vivo model for the study of antibiotic-induced release of endotoxin from gram-negative bacteria is described. The model uses the chronically colonized urinary tracts of patients whose spinal cords have been injured. At baseline, the organisms were present in the range of 1 x 10(3) to 2 x 10(7) CFU/ml, and the concentration of endotoxin ranged from 2 x 10(-1) to 1 x 10(3) ng/ml in 44 studies. In 10 control studies, the concentration of endotoxin and the numbers of viable gram-negative bacteria over time changed by an average of less than 0.15 log10 units from the baseline values. At 2 h after antibiotic administration, the average decrease in CFU was 0.93 log10 units, and because antibiotics cause the release of endotoxin, an average increase in endotoxin concentration of 0.59 log10 units was noted in 21 studies with susceptible bacteria. Similar changes in response to antibiotic exposure were seen in studies with susceptible Pseudomonas bacteria in comparison with those seen in studies with susceptible members of the family Enterobacteriaceae. These results provide evidence that this novel model may be useful for comparing the effects of antibiotics with different modes of action, both as single agents and in combination, on the concentration of endotoxin in relation to changes in the numbers of bacteria, under conditions of bacterial replication and antibiotic exposure more closely resembling those found in vivo than is possible in other models.
Internal ID Number: 1804012
URI: http://ahro.austin.org.au/austinjspui/handle/1/10465
URL: http://www.ncbi.nlm.nih.gov/pubmed/1804012
Type: Journal Article
Subjects: Anti-Bacterial Agents.pharmacology
Bacteriuria.complications.metabolism
Chronic Disease
Ciprofloxacin.pharmacology
Colony Count, Microbial
Endotoxins.metabolism
Enterobacteriaceae.drug effects
Gentamicins.urine
Humans
Lactams
Limulus Test
Models, Biological
Pseudomonas.drug effects
Spinal Cord Injuries.complications
Urinary Tract Infections.microbiology
Appears in Collections:Journal articles

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