Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10167
Title: Lentiviral expression of CTLA4Ig inhibits primed xenogeneic lymphocyte proliferation and cytokine responses.
Authors: Mulley, William R;Wee, Janet L;Christiansen, Dale;Milland, Julie;Ierino, Francesco L;Sandrin, Mauro S
Affiliation: Molecular Immunogenetics Laboratory, The Austin Research Institute, Austin Health, Heidelberg, Vic., Australia.
Issue Date: 1-May-2006
Citation: Xenotransplantation; 13(3): 248-52
Abstract: Co-stimulatory blockade is known to inhibit lymphocyte responses and to prolong allograft and xenograft survival. The present study examines the effect of transgenic expression of cytotoxic T lymphocyte-associated molecule-4 immunoglobulin (CTLA4Ig) by a porcine endothelial cell line (PIEC) transduced by a lentiviral vector, on primed xenogeneic T-cell proliferative and cytokine responses.Splenocytes from mice primed with PIEC were used as responder cells in a secondary proliferative assay. CTLA4Ig transduced and wild-type PIEC were used as stimulator cells. Responder cells were assayed for proliferation and cytokine production.Proliferation was profoundly inhibited by CTLA4Ig transduced cells compared with control cells. Cytokine analysis by enzyme linked immunospot demonstrated that production of interferon-gamma, IL4 (interleukin 4) and IL10 was inhibited by CTLA4Ig transduced cells compared with control cells.CTLA4Ig inhibited primed indirect xenogeneic T-cell proliferative and cytokine responses in vitro. Expression of immunomodulatory molecules by xenogeneic tissues has potential therapeutic applications for future xenotransplantation.
Internal ID Number: 16756567
URI: http://ahro.austin.org.au/austinjspui/handle/1/10167
DOI: 10.1111/j.1399-3089.2006.00297.x
URL: http://www.ncbi.nlm.nih.gov/pubmed/16756567
Type: Journal Article
Subjects: Animals
Animals, Genetically Modified
Cell Line
Cytokines.analysis
Endothelium, Vascular
Genetic Vectors
Immunoconjugates.genetics
Lentivirus.genetics
Lymphocyte Activation
Lymphocyte Transfusion
Mice
Mice, Inbred BALB C
Spleen.immunology
Swine
T-Lymphocytes.immunology
Transplantation, Heterologous.immunology
Appears in Collections:Journal articles

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