Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10163
Title: Alteration of the Fc gamma RIIa dimer interface affects receptor signaling but not ligand binding.
Authors: Powell, Maree S;Barnes, Nadine C;Bradford, Tessa M;Musgrave, Ian F;Wines, Bruce D;Cambier, John C;Hogarth, P Mark
Affiliation: The Macfarlane Burnet Institute for Medical Research and Public Health Limited, Austin Health, Heidelberg, Victoria, Australia
Issue Date: 15-Jun-2006
Citation: Journal of Immunology (baltimore, Md. : 1950); 176(12): 7489-94
Abstract: The aggregation of cell surface FcRs by immune complexes induces a number of important Ab-dependent effector functions. However, despite numerous studies that examine receptor function, very little is known about the molecular organization of these receptors within the cell. In this study, protein complementation, mutagenesis, and ligand binding analyses demonstrate that human FcgammaRIIa is present as a noncovalent dimer form. Protein complementation studies found that FcgammaRIIa molecules are closely associated. Mutagenesis of the dimer interface, as identified by crystallographic analyses, did not affect ligand binding yet caused significant alteration to the magnitude and kinetics of receptor phosphorylation. The data suggest that the ligand binding and the dimer interface are distinct regions within the receptor, and noncovalent dimerization of FcgammaRIIa may be an essential feature of the FcgammaRIIa signaling cascade.
Internal ID Number: 16751395
URI: http://ahro.austin.org.au/austinjspui/handle/1/10163
URL: http://www.ncbi.nlm.nih.gov/pubmed/16751395
Type: Journal Article
Subjects: Animals
Antigens, CD.genetics.metabolism.physiology
Binding Sites.genetics.immunology
CHO Cells
Cricetinae
Cricetulus
Dimerization
Down-Regulation.genetics
Humans
Immunoglobulin G.metabolism
Ligands
Methotrexate.metabolism
Mutagenesis, Site-Directed
Peptide Fragments.biosynthesis.genetics.metabolism
Phosphorylation
Proline.genetics
Receptors, IgG.genetics.metabolism.physiology
Serine.genetics
Signal Transduction.genetics.immunology
Tetrahydrofolate Dehydrogenase.genetics.metabolism
Appears in Collections:Journal articles

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