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Title: Kupffer cell function during the erythocytic stage of malaria.
Authors: Murthi, Padma;Kalionis, Bill;Ghabrial, Hany;Dunlop, Marjorie E;Smallwood, R A;Sewell, Richard B
Affiliation: Department of Medicine, University of Melbourne, Austin and Repatriation Medical Center, Melbourne, Victoria, Australia
Issue Date: 1-Jan-2006
Citation: Journal of Gastroenterology and Hepatology; 21(1 Pt 2): 313-8
Abstract: Previous studies using isolated perfused rat liver in vivo have suggested that during the erythrocytic phase of malaria infection, overall phagocytosis by Kupffer cells is enhanced. The aim of the present study was to further investigate the individual phagocytic capacity and prostaglandin E(2) (PGE(2)) secretion of isolated Kupffer cells in vitro, and the immunohistochemical characteristics of Kupffer cells in vivo.Malaria was induced in male Sprague-Dawley rats (n = 12) by inoculation with parasitized red cells containing Plasmodium berghei. Kupffer cells were isolated by centrifugal elutriation.A significantly increased yield of Kupffer cells was obtained from malaria-infected livers compared to controls (36.7 +/- 4.5 vs 11.8 +/- 1.1 x10(6) cells, P < 0.0001, n = 12). There was an increased internalization by phagocytosis of [(3)H]-BSA latex microspheres after 60 min in malaria-infected Kupffer cells compared to controls (65.05 +/- 1.5 vs 48.6 +/- 0.7, P < 0.001, n = 12). PGE(2) secretion into the cell culture medium was significantly suppressed in malaria-infected Kupffer cells compared to controls (1167 +/- 88 vs 4537 +/- 383 pg per 10(6) cells, P < 0.001, n = 5). Staining of ED1, ED2 and PCNA was greater in malaria-infected livers compared to control.The results indicate that the number of Kupffer cells is significantly increased and their phagocytic activity on a cell-by-cell basis is enhanced during the erythrocytic stage of malaria.
Internal ID Number: 16460493
DOI: 10.1111/j.1440-1746.2006.04192.x
Type: Journal Article
Subjects: Animals
Cell Count
In Vitro Techniques
Kupffer Cells.parasitology.physiology.secretion
Plasmodium berghei
Rats, Sprague-Dawley
Appears in Collections:Journal articles

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