Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10067
Title: Raft localisation of FcgammaRIIa and efficient signaling are dependent on palmitoylation of cysteine 208.
Authors: Barnes, Nadine C;Powell, Maree S;Trist, Halina M;Gavin, A L;Wines, B D;Hogarth, P Mark
Affiliation: Helen Macpherson Smith Trust Inflammatory Disease Laboratory, The Austin Research Institute, Austin Health, Studley Road, Heidelberg, Vic. 3084, Australia.
Issue Date: 5-Dec-2005
Citation: Immunology Letters 2005; 104(1-2): 118-23
Abstract: Ligand-dependent aggregation of FcgammaRIIa initiates multiple biochemical processes including the translocation to detergent resistant membrane domains (DRMs) and receptor tyrosine phosphorylation. Palmitoylation of cysteine residues is considered to be one process that assists in the localisation of proteins to DRMs. Within the juxtamembrane region of FcgammaRIIa there is cysteine residue (C208) that we show to be palmitoylated. Mutation of this cysteine residue results in the disruption of FcgammaRIIa translocation to DRMs as empirically defined by insolubility at high Triton X-100 concentrations. This study also demonstrates that the lack of lipid raft association diminishes FcgammaRIIa signaling as measured by receptor phosphorylation and calcium mobilisation functions suggesting that FcgammaRIIa signaling is partially dependent on lipid rafts.
Internal ID Number: 16375976
URI: http://ahro.austin.org.au/austinjspui/handle/1/10067
DOI: 10.1016/j.imlet.2005.11.007
URL: http://www.ncbi.nlm.nih.gov/pubmed/16375976
Type: Journal Article
Subjects: Animals
Antigens, CD.analysis.genetics.metabolism
B-Lymphocytes.immunology
Calcium Signaling
Cell Line, Tumor
Cysteine.genetics.metabolism
Humans
Membrane Microdomains.chemistry.drug effects.metabolism
Mice
Mutation
Octoxynol.pharmacology
Palmitates.metabolism
Phosphorylation
Protein Processing, Post-Translational.drug effects
Receptors, IgG.analysis.genetics.metabolism
Tyrosine.metabolism
Appears in Collections:Journal articles

Files in This Item:
There are no files associated with this item.


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.