Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10004
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dc.contributor.authorAndrikopoulos, Sofianosen
dc.contributor.authorMassa, Christine Men
dc.contributor.authorAston-Mourney, Kathrynen
dc.contributor.authorFunkat, Alexandraen
dc.contributor.authorFam, Barbara Cen
dc.contributor.authorHull, Rebecca Len
dc.contributor.authorKahn, Steven Een
dc.contributor.authorProietto, Josephen
dc.date.accessioned2015-05-15T23:18:51Z
dc.date.available2015-05-15T23:18:51Z
dc.date.issued2005-10-01en
dc.identifier.citationThe Journal of Endocrinology; 187(1): 45-53en
dc.identifier.govdoc16214940en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/10004en
dc.description.abstractThe increasing production of genetically-modified mouse models has necessitated studies to determine the inherent physiological characteristics of commonly used mouse strains. In this study we examined insulin secretory function in response to an intravenous bolus of glucose or glucose plus arginine in anesthetized C57BL/6, DBA/2 and 129T2 mice fed either a control or high fat diet for 6 weeks. The results show that 129T2 mice had higher fasting plasma glucose levels and lower fasting plasma insulin levels compared with C57BL/6 and DBA/2 mice regardless of diet. Furthermore, 129T2 mice were glucose intolerant and secreted significantly less insulin in response to glucose and glucose plus arginine irrespective of diet compared with the other two strains of mice. DBA/2 mice hypersecreted insulin in response to glucose and glucose plus arginine compared with C57BL/6 and 129T2 mice. Moreover while first phase insulin secretion was appropriately increased in response to the high fat diet in C57BL/6 and 129T2 mice, this was not the case for DBA/2 mice. Mean islet area was decreased in response to a high fat diet in DBA/2 mice, while there was no dietary effect on the other two strains. This study highlights the inherent genetic differences that exist among seemingly normal strains of mice that are commonly used to make transgenic and knockout mice. Understanding these differences will provide researchers with the information to choose the appropriate genetic background on which to express their particular genetic alteration.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherArginine.diagnostic useen
dc.subject.otherBreedingen
dc.subject.otherDietary Fats.administration & dosageen
dc.subject.otherFastingen
dc.subject.otherGlucose.diagnostic useen
dc.subject.otherInsulin.blood.secretionen
dc.subject.otherIslets of Langerhans.cytology.drug effects.secretionen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred C57BLen
dc.subject.otherMice, Inbred DBAen
dc.subject.otherMice, Inbred Strains.physiologyen
dc.subject.otherSpecies Specificityen
dc.titleDifferential effect of inbred mouse strain (C57BL/6, DBA/2, 129T2) on insulin secretory function in response to a high fat diet.en
dc.typeJournal Articleen
dc.identifier.journaltitleThe Journal of Endocrinologyen
dc.identifier.affiliationUniversity of Melbourne, Department of Medicine, Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australiaen
dc.identifier.doi10.1677/joe.1.06333en
dc.description.pages45-53en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/16214940en
dc.type.austinJournal Articleen
local.name.researcherProietto, Joseph
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptMedicine (University of Melbourne)-
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