Effects of Amylin Deficiency on Trabecular Bone in Young Mice Are Sex-Dependent
- R. A. Davey,
- A. J. Moore,
- M. W. S. Chiu,
- A. J. Notini,
- H. A. Morris,
- J. D. Zajac
- … show all 6 hide
Rent the article at a discount
Rent now* Final gross prices may vary according to local VAT.
Get AccessAbstract
Amylin deficiency in mice results in late-onset osteopenia. Sex differences have been identified in insulin secretion in Amylin-overexpressing transgenic mice, suggesting a possible interaction of sex steroids, growth factors, or cytokines and amylin. The aim of the current study was to compare the effects of amylin deficiency on bone in young and adult male and female mice. The metaphyses of the distal femora from male and female Amylin-deficient mice at 4, 6, and 26 weeks of age were assessed by bone histomorphometry. Femoral length was increased in Amylin-deficient male mice compared to wild-type (WT) mice at 26 weeks of age (P < 0.005) but not in females. This was associated with an increase in growth plate height in Amylin-deficient males at 4 (P < 0.01) and 6 (P < 0.05) weeks of age. Furthermore, young Amylin-deficient males had decreased trabecular number at 4 weeks of age (P < 0.05) and increased trabecular thickness at 4 and 6 weeks of age (P < 0.05) compared to WT mice, with no net change in trabecular bone volume. These effects of amylin deficiency were not observed in female mice. In conclusion, this study demonstrates that amylin deficiency exerts effects on bone during growth that are sex-dependent and suggest a possible interaction between amylin and testosterone, growth factors, or cytokines to regulate bone cell metabolism.
- Dacquin, R, Davey, RA, Laplace, C, Levasseur, R, Morris, HA, Goldring, SR, Gebre-Medhin, S, Galson, DL, Zajac, JD, Karsenty, G (2004) Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo. J Cell Biol 164: pp. 509-514 CrossRef
- Christopoulos, G, Perry, KJ, Morfis, M, Tilakaratne, N, Gao, Y, Fraser, NJ, Main, MJ, Foord, SM, Sexton, PM (1999) Multiple amylin receptors arise from receptor activity-modifying protein interaction with the calcitonin receptor gene product. Mol Pharmacol 56: pp. 235-242
- Muff, R, Born, W, Fischer, JA (1995) Calcitonin, calcitonin gene-related peptide, adrenomedullin and amylin: homologous peptides, separate receptors and overlapping biological actions. Eur J Endocrinol 133: pp. 17-20
- Muff, R, Born, W, Fischer, JA (1995) Receptors for calcitonin, calcitonin gene related peptide, amylin, and adrenomedullin. Can J Physiol Pharmacol 73: pp. 963-967
- Hay, DL, Christopoulos, G, Christopoulos, A, Sexton, PM (2004) Amylin receptors: molecular composition and pharmacology. Biochem Soc Trans 32: pp. 865-867 CrossRef
- Cooper, GJ (1994) Amylin compared with calcitonin gene-related peptide: structure, biology, and relevance to metabolic disease. Endocr Rev 15: pp. 163-201 CrossRef
- Alam, AS, Moonga, BS, Bevis, PJ, Huang, CL, Zaidi, M (1993) Amylin inhibits bone resorption by a direct effect on the motility of rat osteoclasts. Exp Physiol 78: pp. 183-196
- Cornish, J, Callon, KE, Cooper, GJ, Reid, IR (1995) Amylin stimulates osteoblast proliferation and increases mineralized bone volume in adult mice. Biochem Biophys Res Commun 207: pp. 133-139 CrossRef
- Cornish, J, Callon, KE, King, AR, Cooper, GJ, Reid, IR (1998) Systemic administration of amylin increases bone mass, linear growth, and adiposity in adult male mice. Am J Physiol Endocrinol Metab 275: pp. E694-E699
- Horcajada-Molteni, MN, Davicco, MJ, Lebecque, P, Coxam, V, Young, AA, Barlet, JP (2000) Amylin inhibits ovariectomy-induced bone loss in rats. J Endocrinol 165: pp. 663-668 CrossRef
- Romero, DF, Bryer, HP, Rucinski, B, Isserow, JA, Buchinsky, FJ, Cvetkovic, M, Liu, CC, Epstein, S (1995) Amylin increases bone volume but cannot ameliorate diabetic osteopenia. Calcif Tissue Int 56: pp. 54-61 CrossRef
- Verchere, CB, D’Alessio, DA, Palmiter, RD, Weir, GC, Bonner-Weir, S, Baskin, DG, Kahn, SE (1996) Islet amyloid formation associated with hyperglycemia in transgenic mice with pancreatic beta cell expression of human islet amyloid polypeptide. Proc Natl Acad Sci USA 93: pp. 3492-3496 CrossRef
- Gebre-Medhin, S, Mulder, H, Pekny, M, Westermark, G, Tornell, J, Westermark, P, Sundler, F, Ahren, B, Betsholtz, C (1998) Increased insulin secretion and glucose tolerance in mice lacking islet amyloid polypeptide (amylin). Biochem Biophys Res Commun 250: pp. 271-277 CrossRef
- Baldock, PA, Moore, RJ, Durbridge, TC, Morris, HA (1999) Comparison of three methods for estimation of bone resorption following ovariectomy in the distal femur and the proximal tibia of the rat. Bone 24: pp. 597-602 CrossRef
- Page, K eds. (1997) Bone and Preparation of Bone Sections. Churchill Livingstone, London
- Pagano, R (1986) Understanding Statistics in the Behavioural Sciences. West Publications, New York
- Ogawa, A, Harris, V, McCorkle, SK, Unger, RH, Luskey, KL (1990) Amylin secretion from the rat pancreas and its selective loss after streptozotocin treatment. J Clin Invest 85: pp. 973-976 CrossRef
- Jamal, H, Bretherton-Watt, D, Suda, K, Ghatei, MA, Bloom, SR (1990) Islet amyloid polypeptide-like immunoreactivity (amylin) in rats treated with dexamethasone and streptozotocin. J Endocrinol 126: pp. 425-429 CrossRef
- Cortright, RN, Collins, HL, Chandler, MP, Lemon, PW, DiCarlo, SE (1996) Diabetes reduces growth and body composition more in male than in female rats. Physiol Behav 60: pp. 1233-1238 CrossRef
- Ostenson, CG, Grill, V, Roos, M (1989) Studies on sex dependency of B-cell susceptibility to streptozotocin in a rat model of type II diabetes mellitus. Exp Clin Endocrinol 93: pp. 241-247 CrossRef
- Takahashi, T, Ogasawara, T, Kishimoto, J, Liu, G, Asato, H, Nakatsuka, T, Uchinuma, E, Nakamura, K, Kawaguchi, H, Takato, T, Hoshi, K (2005) Synergistic effects of FGF-2 with insulin or IGF-I on the proliferation of human auricular chondrocytes. Cell Transplant 14: pp. 683-693
- Taylor, AM, Dunger, DB, Preece, MA, Holly, JM, Smith, CP, Wass, JA, Patel, S, Tate, VE (1990) The growth hormone independent insulin-like growth factor-I binding protein BP-28 is associated with serum insulin-like growth factor-I inhibitory bioactivity in adolescent insulin-dependent diabetics. Clin Endocrinol (Oxf) 32: pp. 229-239
- Beamer, WG, Donahue, LR, Rosen, CJ, Baylink, DJ (1996) Genetic variability in adult bone density among inbred strains of mice. Bone 18: pp. 397-403 CrossRef
- Title
- Effects of Amylin Deficiency on Trabecular Bone in Young Mice Are Sex-Dependent
- Journal
-
Calcified Tissue International
Volume 78, Issue 6 , pp 398-403
- Cover Date
- 2006-06-01
- DOI
- 10.1007/s00223-005-0286-2
- Print ISSN
- 0171-967X
- Online ISSN
- 1432-0827
- Publisher
- Springer-Verlag
- Additional Links
- Topics
- Keywords
-
- Animal model
- Rodent
- Knockout/knockin
- Bone histomorphometry
- Industry Sectors
- Authors
-
- R. A. Davey (1)
- A. J. Moore (2)
- M. W. S. Chiu (1)
- A. J. Notini (1)
- H. A. Morris (2)
- J. D. Zajac (1)
- Author Affiliations
-
- 1. Department of Medicine, University of Melbourne, Austin Health, Heidelberg, Victoria, Australia
- 2. Hanson Institute, Adelaide, South Australia, Australia